# Notes: Data obtained In-Cell Western (see Methods and Materials).
# Notes: All data normalized between 0 and 100.
# Notes: 0 indicates basal value; 100 indicates peak value for one signal across all conditions.
# Notes: 0 signal in pErbB1 indicate undetectable levels.

Condition: High (5e-9 M) EGF Measured Signal: pAkt
Time (min)	Signal	Standard Error
0	3.6	3.5
2.5	0.99	2.6
5	21	6.8
7.5	26	8.4
10	22	2.8
15	18	5.5
30	0	1.7
45	0	2.4
60	0	7
1.2e+02	0	3.1

Condition: High (5e-9 M) EGF Measured Signal: pErbB1
Time (min)	Signal	Standard Error
0	5.6	6
2.5	28	9.6
5	44	20
7.5	46	17
10	55	8.6
15	70	9.9
30	1e+02	23
45	62	18
60	70	12
1.2e+02	88	14

Condition: High (5e-9 M) EGF Measured Signal: pERK
Time (min)	Signal	Standard Error
0	1.3	1.2
2.5	34	2.1
5	75	6.9
7.5	72	7.5
10	79	15
15	1e+02	12
30	1e+02	24
45	64	3.3
60	59	4.6
1.2e+02	27	2.6

Condition: High (5e-9 M) HRG Measured Signal: pAkt
Time (min)	Signal	Standard Error
0	-3.5	2.8
2.5	30	31
5	65	27
7.5	80	32
10	85	32
15	97	29
30	92	19
45	72	24
60	90	23
1.2e+02	89	23

Condition: High (5e-9 M) HRG Measured Signal: pErbB1
Time (min)	Signal	Standard Error
0	0	0
2.5	0	0
5	0	0
7.5	0	0
10	0	0
15	0	0
30	0	0
45	0	0
60	0	0
1.2e+02	0	0

Condition: High (5e-9 M) HRG Measured Signal: pERK
Time (min)	Signal	Standard Error
0	-2.7	3.3
2.5	13	6.2
5	21	11
7.5	13	7.8
10	11	7.5
15	14	3.4
30	13	6.8
45	11	3
60	15	2
1.2e+02	0.17	3.9

Condition: Low (1e-11 M) EGF Measured Signal: pAkt
Time (min)	Signal	Standard Error
0	0.21	4.2
2.5	0	4.5
5	4.1	6.5
7.5	12	5
10	11	5.4
15	7.7	5.8
30	0	3.1
45	0	2.5
60	0	2.5
1.2e+02	0	2.2

Condition: Low (1e-11 M) EGF Measured Signal: pErbB1
Time (min)	Signal	Standard Error
0	0	0
2.5	0	0
5	0	0
7.5	0	0
10	0	0
15	0	0
30	0	0
45	0	0
60	0	0
1.2e+02	0	0

Condition: Low (1e-11 M) EGF Measured Signal: pERK
Time (min)	Signal	Standard Error
0	0	2.3
2.5	7.1	0.22
5	19	6.9
7.5	5.8	2
10	0	2.1
15	1.1	6.7
30	0.026	5.4
45	1.4	6.2
60	2.9	5
1.2e+02	0	5

Condition: Low (1e-10 M) HRG Measured Signal: pAkt
Time (min)	Signal	Standard Error
0	-0.25	4.8
2.5	19	39
5	56	43
7.5	71	46
10	89	59
15	1e+02	46
30	91	39
45	71	26
60	85	40
1.2e+02	92	29

Condition: Low (1e-10 M) HRG Measured Signal: pErbB1
Time (min)	Signal	Standard Error
0	0	0
2.5	0	0
5	0	0
7.5	0	0
10	0	0
15	0	0
30	0	0
45	0	0
60	0	0
1.2e+02	0	0

Condition: Low (1e-10 M) HRG Measured Signal: pERK
Time (min)	Signal	Standard Error
0	1.7	1.6
2.5	19	12
5	28	7.6
7.5	18	6.4
10	13	4.4
15	18	5.9
30	18	3.2
45	10	6.3
60	15	6.7
1.2e+02	3.6	5.2