Paul Huang

Biosketch

BSc. (Hons) Biotechnology, Imperial College London UK, 2004
Diploma of Associate of the Royal College of Science UK, 2004

Graduate Student, Biological Engineering, MIT
Advisors: Forest M. White & Peter K. Sorger

Research Interests

The focus of my research is to understand how protein phosphorylation-mediated signaling networks drive biological responses to cellular stimulation. One major goal of this research is to answer questions pertinent to oncogenic signaling in various cancers.

To achieve a greater understanding of signaling networks with associated temporal dynamics, our lab has developed a mass spectrometric approach to simultaneously quantify tyrosine phosphorylation on specific sites on a large number of key signaling proteins in a multiplexed manner. This involves the use of hybrid quadrupole time-of-flight mass spectrometers coupled with LC-MS/MS and affinity chromatography [1].

Once sufficient data has been collected and analyzed, computational tools will be used to relate the mass spectrometric data on signal transduction networks to phenotypic responses and generate experimentally testable predictions.

This work is funded by the NCI Integrative Cancer Biology Program. Collaborators on this project include the Lauffenburger and Housman Labs.

1. Zhang, Y., et al., Time-resolved mass spectrometry of tyrosine phosphorylation sites in the EGF receptor signaling network reveals dynamic modules. Mol Cell Proteomics, 2005.