Claudia Donnet

My main focus is the analysis of phosphotyrosine signaling networks applied to cancer research.

Primary Collaborations:

Paul Jasper, Sorger lab, MIT
Matthew Lazzara, Sorger Lab, MIT
Gordon Lu, Sorger Lab, MIT

Education

Philosophical Doctor, Biochemistry/Biophysics
University of Buenos Aires, Argentina
Research Topic: Functional effects of chemical modification of the plasma membrane Ca-ATPase lysine residues
Advisor: Juan Pablo FC Rossi

Biochemist (equivalent to Bachelor and Master)
School of Pharmacy and Biochemistry, University of Buenos Aires, Argentina

Honors / Awards

School of Biochemistry Award to the Best Thesis of the Year

Previous Research Experience

Post-Doctoral Research Fellow at the Massachusetts General Hospital/ Harvard Medical School, Boston, MA
Advisor: Kathleen Sweadner
I worked in several projects that focus on studying the structure, function and regulation of the Na,K-ATPase.

Publications

• Arystarkhova, E., Donnet, C., Muñoz-Matta, A., Corey, S. and Sweadner, K.J. “Multiplicity of expression of FXYD proteins in mammalian cells: dynamic exchange of phospholemman and gamma subunit in response to stress”. (submitted to Am.J.Physiol., 6/2006)

• L.G. Jia, Donnet, C., Bogaev, R.C., Blatt R.J., McKinney C.E., Day K.H., Berr S.S., Jones L.R., Moorman J.R., Sweadner K.J., and Tucker A.L. “The role of phospholemman in the murine heart: Evidence for cardiac hypertrophy and reduced Na,K+ATPase activity in phospholemman–deficient mice”, Am. J. Physiol. Heart Circ. Physiol. (2005) 288: H1982-H1988.

• Feschenko, M.S., Donnet, C., Wetzel, R.K., Asinovski, N.K., Jones, L. and Sweadner, K.J. " Phospholemman, a single-span membrane protein, is an accessory protein of Na,K-ATPase in cerebellum and choroid plexus " J. Neurosci. (2003) 23:2161-2169.

• Donnet, C. & Sweadner K.J. "The Mechanism of Na-K Interaction on Na,K-ATPase". Ann. N.Y. Acad. Sci. (2003) 986: 249-251.

• Sweadner, K.J., Arystarkhova, E. Donnet, C. & Wetzel, R.K. "FXYD proteins as regulators of theNa,K-ATPase in the kidney". Ann. N.Y. Acad. Sci. (2003) 986: 382-387.

• Reyes A.M., Bustamante F., Rivas C.I., Ortega M., Donnet, C., Rossi J.P., Fischbarg J. and Vera J.C. "Nicotinamide is not a Substrate of the Facilitative Hexose Transporter GLUT1" Biochemistry (2002) 41:8075-8081.

• Arystarkhova E., Donnet, C. and K. J. Sweadner "Differential regulation of renal Na,K-ATPase by splice variants of the gamma subunit". J Biol Chem. (2002) 277:10162-10172.

• Sweadner, K.J. and Donnet, C. "Structural similarities of Na,K-ATPase and SERCA, the Ca(2+)-ATPase of the sarcoplasmic reticulum" Biochem J. (2001) 356:685-704.

• Donnet, C., Arystarkhova, E. and K. J. Sweadner "Thermal Denaturation of the Na,K-ATPase Provides Evidence for Oligomeric Interaction and Subunit Association with the C-terminal Domain" J. Biol. Chem. (2001) 276: 7357-7365.

• Donnet, C., A.J. Caride, S.A. Talgham & J. P. F. C. Rossi "Chemical modification reveals involvement of different sites for nucleotide-analogs in the phosphatase activity of the red cell calcium pump" J. Membr. Biol. (1998) 163:217-224.

• Donnet, C., A. J. Caride, S.A. Talgham & J.P.F.C. Rossi (1996) "Involvement of different sites for nucleotide-analogs in the phosphatase activity of the red cell calcium pump". In "The Sodium Pump" (Garrahan, P.J., Beauge, L.A. and Gadsby, D.C. eds) Ann. New York Acad. Science.

• Donnet, C. (1994). "Kinetic effects of lysine modification in the calcium pump of plasma membrane". PhD. Thesis, University of Buenos Aires, Argentina.

• Donnet, C., A. J. Caride, H. N. Fernández y J.P.F.C. Rossi "Acetylation with succinimidyl acetate affects both the catalytic site and the regulation of the red cell calcium pump" Biochem. J. (1994) 302: 133-140.

• Donnet, C., A. J. Caride, H.N. Fernández y J.P.F.C. Rossi "Differential reactivity of lysine residues of the red cell Ca2+ pump involved in the E1-E2 conformational equilibrium" Biochem. J.(1991) 279, 121-127.

• Donnet, C., S. Medrano, M. Otero, E.L.M. Ochoa y J.P.F.C. Rossi "Characteristics of a presynaptic plasma membrane Ca2+-ATPase activity from electric organ" Biochim. Biophys. Acta (1990) 1030, 195-202.