Ale Wolf

Education

1999 Bachelor in Engineering Sciences Degree in Biotechnology, Universidad de Chile.

2000 European Community alpha scholarship for graduate students. Lund University, Sweden

2001 Civil Engineering Degree in Biotechnology, Universidad de Chile.

2001 Master in Engineering Sciences Degree in Chemical Engineering, Universidad de Chile. (Research performed at Lund University, Sweden )

2007 PhD in Biological Engineering, MIT. Advisors: Forest White and Douglas Lauffenburger

Research in Computational and Systems Biology

Development of new quantitative mass spectrometry techniques to analyze protein phosphorylation in cell signaling. Applications are mainly focused to the study of breast cancer model systems and computational analysis is done to correlate our protein phosphorylation data set to phenotypic characteristics of the cells.

Primary Collaborators

Neil Kumar, Lauffenburger Lab, MIT Sampsa Hautaniemi, Lauffenburger Lab, MIT Yi Zhang, White Lab, MIT

Selected Publications

1) Beggren, K., Wolf, A., Asenjo, J.A., Andrews, B., and Tjerneld, F. “The surface exposed amino acid residues of monomeric proteins determine the partitioning in aqueous two-phase systems.”, Biochemica et Biophysica Acta. 1596, 253-268 (2002).

2) Wolf-Yadlin A., Zhang Y., Ross, P.L., Papin D.J., Rush J., Lauffenburger, D.A., White F.M. “ Time-resolved mass spectrometry of tyrosine phosphorylation sites in the EGF receptor signaling network reveals dynamic modules.” Molecular Cellular Proteomics. 4(9), 1240-1250 (2005).

3) Wolf-Yadlin, A., Zhang, Y., and White, F.M. “Quantitative Proteomic Analysis of Phosphotyrosine-Mediated Cellular Signaling Networks”, submitted to Methods in Molecular Biology. (2005)